Abstract: Background: Pressure Ulcer (PU) is a serious health condition that exhaust health care professionals, especially nurses. as prolonged hospitalization is a leading risk factor; however, PU is a preventable injury. Furthermore, Nurses have a crucial role in PU prevention and management, mainly by applying PU prevention and management clinical guidelines. The literature suggests that such implementation is affected by nurses' perception of PU. This study focused on three components of nurses' perception, including (1) knowledge regarding PU, (2) attitude toward PU, and (3) perceived barriers to PU prevention. Aim: The study aimed to identify the level of knowledge and attitude toward PU Prevention and the perception of barriers to implementing PU prevention guidelines among nurses at Salmaniya Medical Complex (SMC). Methodology: The study followed a descriptive cross-sectional design. The study was conducted at SMC and included 132 nurses from critical and non-critical working areas. A simple randomization method was used to select the study sample, and three calibrated questionnaires were used to assess the study variables: (1) Pressure Ulcer Knowledge Assessment Tool 2.0 (PUKAT 2.0), (2) Attitude towards Pressure Ulcer Prevention instrument (APuP), and (3) Pressure Injury Prevention Barriers (PIPB). Results: Results showed that 12.1 % (n=16) of nurses had satisfactory knowledge about PU, and about 65.15 % (n=86) demonstrated a positive attitude toward PU prevention. There was no statistically significant difference between critical and non-critical care nurses regarding knowledge of PU (p-value = 0.84) or attitude toward PU (p-value =0.45). The lack of devices was the most reported barrier to PU prevention, followed by uncooperative patient families. Conclusion: Nurses showed inadequate knowledge regarding PU, especially in terms of PU prevention; however, nurses have demonstrated a positive attitude toward PU and reported a low number of barriers to PU prevention; subsequently, a structured training program may elevate nurses' knowledge and improve care. Keywords: Pressure Ulcer; Knowledge; Attitudes; Prevention & control; Nurses.