ABSTRACT :

Breast cancer stands as the leading cause of cancer-related fatalities among women globally,presenting a diverse landscape of histological and molecular c characteristics, treatmentresponse, and prognosis. This investigation examined the impact of soyasaponin IV, a naturalproduct, on mice carrying Ehrlich’s ascites carcinoma (EAC), exploring its potential in bloster-ing the hosts’ defense against cancer. The isolated compounds underwent scrutiny for theircytotoxic effects on breast cancer cell line, with Doxorubicin as the reference standard.Notably, Soyasaponin IV exhibited the highest cytotoxicity. Evaluation of its in vivo antitumorefficacy against EAC revealed a substantial reduction in tumor weight and volume, accompa-nied by improved histological features of tumor cells. EAC-induced abnormalities, includingelevated levels of Malondialdehyde (MDA), vascular endothelial growth factor (VEGF), andnuclear factor-kappa B (NF-jB) were all shown to be upregulated by EAC, while glutathione(GSH), were mitigated in a dose-dependent manner by soyasaponin IV treatment.Soyasaponin IV emerged as a promising strategy for suppressing EAC by impeding the NF-jB and VEGF pathways